Huang, Hsiou-Cheng
黃秀珍 教授
Email: [email protected]
地址:中興大學食品暨生物科技大樓6F-606
Office phone: (04) 2284-0328 ext.631
Lab phone: (04) 2284-0328 ext.632
專長
植物病理學、植物病原細菌學、微生物遺傳學
學經歷
- B.S. 國立中興大學 植病系 1980
- M.S. 國立中興大學 植病所 1982
- Ph.D. Cornell University Plant pathology 1991
- 1991 PDF University of California, Berkely
- 1991-1998 副教授 國立中興大學遺傳中心
- 1998-1998 副教授 國立中興大學農科所
- 1999-present 教授 國立中興大學生物科技所
研究興趣
病原細菌分子致病機制探討
本研究室的研究內容主要以分子遺傳學探討植物病原菌Pseudomonas syringae pv. averrhoi (楊桃細菌性斑點病菌) 及Acidovorax avenae subsp citrulli (瓜類果斑病菌) 的Hrp/Hrc/Hop/Avr等蛋白質,在與寄主植物發生交互作用所扮演的角色。 一群Hrp/Hrc蛋白質主要是構成細菌的第三型分泌系統,而此分泌系統可將細菌所製造的有效蛋白 (effectors, eg. Hop proteins) 及harpin等蛋白分泌於胞外,甚至傳送有效蛋白至植物細胞質內,改變寄主的生理作用,以致促進病菌的繁殖及產生病徵,最終導致寄主死亡。另外也評估利用病菌所產生的harpin蛋白,以轉殖植物的方式,促進植物的抗病性之潛力。
Molecular mechanisms of phytobacterial pathogenesis
The aim of our research is to investigate biological functions of the Hrp/Hrc/Hop/Avr/Hpa proteins ofPseudomonas syringae pv. averrhoi (a pathogen of starfruit) and Acidovorax avenae subsp citrulli (a pathogen of cucurbit) on pathogenesis. These pathogens contain a Type III secretion apparatus encoded by hrc/hrp genes, which can secrete harpins into media and translocate hop/hpa/avr-coding effectors into their host cells. Those Hop/Hpa/Avr proteins can manipulate the biochemical or physiological reactions of their infected plants, resulting in susceptible or resistant responses. Harpin proteins produced by phytobacteria can induce the resistant response, therefore, we now investigate the potential application of harpin proteins in inducing resistance to bacterial diseases of economic crops.
本研究室的研究內容主要以分子遺傳學探討植物病原菌Pseudomonas syringae pv. averrhoi (楊桃細菌性斑點病菌) 及Acidovorax avenae subsp citrulli (瓜類果斑病菌) 的Hrp/Hrc/Hop/Avr等蛋白質,在與寄主植物發生交互作用所扮演的角色。 一群Hrp/Hrc蛋白質主要是構成細菌的第三型分泌系統,而此分泌系統可將細菌所製造的有效蛋白 (effectors, eg. Hop proteins) 及harpin等蛋白分泌於胞外,甚至傳送有效蛋白至植物細胞質內,改變寄主的生理作用,以致促進病菌的繁殖及產生病徵,最終導致寄主死亡。另外也評估利用病菌所產生的harpin蛋白,以轉殖植物的方式,促進植物的抗病性之潛力。
Molecular mechanisms of phytobacterial pathogenesis
The aim of our research is to investigate biological functions of the Hrp/Hrc/Hop/Avr/Hpa proteins ofPseudomonas syringae pv. averrhoi (a pathogen of starfruit) and Acidovorax avenae subsp citrulli (a pathogen of cucurbit) on pathogenesis. These pathogens contain a Type III secretion apparatus encoded by hrc/hrp genes, which can secrete harpins into media and translocate hop/hpa/avr-coding effectors into their host cells. Those Hop/Hpa/Avr proteins can manipulate the biochemical or physiological reactions of their infected plants, resulting in susceptible or resistant responses. Harpin proteins produced by phytobacteria can induce the resistant response, therefore, we now investigate the potential application of harpin proteins in inducing resistance to bacterial diseases of economic crops.
期刊論文
- Wei C-F, Hsu S-T, Deng W-L, Wen Y-D, and Huang H-C*. 2012. Plant innate immunity induced by flagellin suppresses the hypersensitive response in non-host plants elicited by Pseudomonas syringae pv. averrhoi. PLoS One. (7):e41056.
- Lin R-H, Peng C-W, Lin Y-C, Peng H-L, and Huang H-C*. 2011. The XopE2 effector protein of Xanthomonas campestris pv. vesicatoria is involved in virulence and in the suppression of the hypersensitive response. Botanical Studies. 52:55-72.
- Tseng J-M, Huang J-R, Huang H-C, Tzen JTC, Chou W-M, and Peng C-C. 2011. Facilitative production of an antimicrobial peptide-royalisin and its antibody via an artificial oil body system. Biotechnol. Prog. 27(1):153-161.
- Deng W-L, Lin Y-C, Lin R-H, Wei C-F, Peng H-L, Huang Y-C, and Huang H-C*. 2010. Effects of galU mutation on Pseudomonas syringae-plant interactions. Mol. Plant-Micro. Interact. 23:1184-1196.
- Chu M-K, Lin L-F, Twu C-S, Lin R-H, Lin Y-C, Hsu S-T, Tzeng K-C, and Huang H-C*. 2010. Unique features of Erwinia chrysanthemi (Dickeya dadantii) RA3B genes involved in the blue indigoidine production. Microbiol. Res. 165(6):483-495.
- Ramos AR, Morello JE, Ravindran S, Deng W-L, Huang H-C, and Collmer A. 2007. Identification of Pseudomonas syringae pv. syringae 61 type III secretion system Hrp proteins that can travel the type III pathway and contribute to the translocation of effector proteins into plant cells. J. Bacteriol. 189:5773-5778.
- Wei C-F, Kvitko BH, Shimizu R, Crabill E, Alfano JR, Lin N-C, Martin GB, Huang H-C, and Collmer A. 2007. Pseudomonas syringae pv. tomato DC3000 mutant lacking the type III effector HopQ1-1 is able to cause disease in the model plant Nicotiana benthamiana. The Plant J. 51:32-46.
- Lin Y-C, Hsu S-T, and Huang H-C*. 2006. Evidence for the interaction between HrpT and HrcC of Pseudomonas syringae pv. syringae 61. Plant Pathol. Bull. (Taiwan) 15:171-185.
- Lin Y-C, Hu Y-M, Hsu S-T, Tzeng K-C, and Huang H-C*. 2006. Cloning and characterization of exchangeable effector locus of Pseudomonas syringae pv. averrhoi, a new pathogen on Averrhoa carambola. Plant Pathol. Bull. (Taiwan) 15:139-152.
- Wei C-F, Deng W-L, and Huang H-C*. 2005. A chaperone-like HrpG protein acts as a suppressor of HrpV in regulation of the Pseudomonas syringae pv. syringae type III secretion system. Mol. Microbiol. 57:520-536
- Chung W-C, Huang H-C, Chiang B-T, and Huang J-W. 2005. Inhibition of Soil-borne Plant Pathogens by the Treatment of Sinigrin and Myrosinases Released from Reconstructed Escherichia coli and Pichia pastoris. Biocontrol Science and Technology (BST) 15:455-465.
- Lin Y-H, Chang S-C, Tsai Y-L, Hsu S-T, Huang H-C, and Tzeng K-C. 2004. Effect of cyanogenic Pseudomonas aeruginosa on the growth of plant bacteria. Plant Pathol. Bull. (Taiwan) 13:211-218.
- Lin Y-H, Hsu S-T, Huang H-C, and Tzeng K-C. 2004. Cloning of plcSR genes from foliar Pseudomonas aeruginosa WFP11r and the role of hemolytic phospholipase C in pathogenesis on plants. Plant Pathol. Bull. (Taiwan) 13:183-190.
- Chiang B-T and Huang H-C*. 2003. Expression of rca gene in Chenopodium quinoa during the hypersensitive response induced by Pseudomonas syringae pv. syringae. Plant Pathol. Bull. (Taiwan) 12:93-102.
國立中興大學生物科技學研究所© 2014 [email protected] TEL:(04)2284-0328 FAX:(04)2285-3527
台中市南區國光路250號生物科技學研究所 No.250 Kuo-Kuang Rd., Taichung, 402 Taiwan, R.O.C.
台中市南區國光路250號生物科技學研究所 No.250 Kuo-Kuang Rd., Taichung, 402 Taiwan, R.O.C.