图片Dr. Hu, Chung-Chi


Hu, Chung-Chi

胡仲祺 教授

Email: [email protected]

地址:中興大學食品暨生物科技大樓6F617室

Office phone:22840328 ext.621

Lab phone:22840328 ext.628

主要教授課程 (含大學部及研究所) 

  • 生物化學
  • 高等生物化學
  • 分子生物學
  • 分子生物學技術與原理
  • 生物資訊學導論
  • 生物資訊學特論

學經歷

  • 學士： 國立中興大學 植物病理系 1985
  • 碩士： 國立中興大學 植物病理研究所 1987
  • 博士： Dept. Plant Pathology, University of Kentucky 1997
  • 博士後研究：Dept. Plant Pathology, University of Kentucky 1998
  • 助理研究員：國立中興大學 遺傳工程中心
  • 助理教授： 國立中興大學 生物科技學研究所
  • 副教授： 國立中興大學 生物科技學研究所(2009.08.01)
  • 教授： 國立中興大學 生物科技學研究所(2016.02.01)

研究項目簡介

  • 胡瓜嵌紋病毒衛星核酸複製及包被機制之探討與衛星核酸載體之研發：
      衛星核酸為病毒之分子寄生物，需要依賴輔助病毒進行其複製、包被、及傳播，但卻與其輔助病毒無核酸序列同源性。許多衛星核酸不帶有任何顯著的轉譯區，然而卻具有調控其輔助病毒之複製及病徵表現的能力，因此其生物活性之表達必須藉由其核酸分子本身與其他相關因子之直接交互作用，且所需之複製、包被等調控訊息亦須存在於此核酸分子上。故衛星核酸為一極佳之材料用以探討其輔助病毒之複製、包被、及病徵表現之機制。且因衛星核酸之分子遠較其輔助病毒為小，亦可在寄主植物上累積至高量，故衛星核酸具有極高的潛力可被發展成為高量表達之基因載體系統，在病毒分子生物學之基礎研究及實際應用上均具有極高的價值。

胡瓜嵌紋病毒為cucumovirusgroup之模式病毒，寄主範圍極廣，包含單、雙子葉植物近一千種，具有極高之經濟重要性。本實驗室自中興大學徐堯煇教授處獲得十九個胡瓜嵌紋病毒株系，之中十五個株系帶有衛星核酸。而其中C株系(CMV-C)之衛星核酸(C-Sat)可減輕其輔助病毒之病徵表現，但M株系(CMV-M)之衛星核酸(M-Sat)則無影響病徵表現之能力。此二衛星核酸皆已被選殖及定序，並可經由T7起動子轉錄出具有感染力之RNA。由於此衛星核酸長度僅有334個核酸，因此可作為一理想之實驗系統以探討病毒或衛星核酸複製、包被所需之基本訊息，而所獲得之結果亦可作為將衛星核酸發展為基因載體系統之依據，且因胡瓜嵌紋病毒之寄主範圍極廣，預期此載體系統將可被廣泛應用於多種經濟作物上。

  • 台灣雙生病毒病害之診測：
       銀葉粉蝨傳播之雙生病毒 (geminiviruses)可危害40種以上蔬菜和纖維作物,發生嚴重時可完全摧毀作物的生產，此病害的發生由熱帶地區逐漸擴展至亞熱帶溫帶,已成全球性的重要植物性病毒病原。在本省已有數種作物及雜草發生類似雙生病毒感染，造成黃化及葉片捲曲的病徵，但這些病害皆有待進一步確定其病原。近期中，南部有些地區蕃茄捲葉病的肆虐，使得蕃茄幾無收成。而其媒介昆蟲(粉蝨類)亦已逐漸適應本省的農作物生態，密度急劇升高。可預見雙生病毒即將躍居台灣農作物之主要病原。利用傳統病毒學的方法鑑定雙生病毒已被證明相當困難，因此本實驗室擬建立分子生物學方法以鑑定本省所發生之雙生病毒病害，並發展分子或血清試劑，提供未來抗病育種或種苗檢疫的基本工具。 

期刊論文

Ａ、期刊論文：

  1. Huang, Y. W., Hu, C. C., Liou, M. R., Chang, B. Y., Tsai, C. H.,Meng, M., Lin, N. S., & Hsu, Y. H. (2012) Hsp90 Interacts Specifically with Viral RNA and Differentially Regulates Replication Initiation of Bamboo mosaic virus and Associated Satellite RNA. PLoS Pathog 8(5): e1002726. doi:10.1371/journal.ppat.1002726.

  2. Chen, T. H,, Chen, T. H., Hu, C. C., Liao J. T., Lee, C. W., Liao, J. W., Lin, M, Y,, Liu, H. J., Wang, M. Y., Lin, N. S., Hsu, Y. H. (2012) Induction of protective immunity in chickens immunized with plant-made chimeric Bamboo mosaic virus particles expressing very virulent Infectious bursal disease virus antigen. Virus Res. 166, 109-15.

  3. Prasanth, K. R., Huang, Y. W., Liou, M. R., Wang, R. Y., Hu, C. C., Tsai, C. H., Meng, M., Lin, N. S. & Hsu, Y. H. 2011. Glyceraldehyde 3-phosphate dehydrogenase negatively regulates the replication of Bamboo mosaic virus and its associated satellite RNA. J Virol. 85, 8829-40.

  4. Shun-Fang Cheng, Ying-Ping Huang, Zi-Rong Wu, Chung-Chi Hu, Yau-Heiu Hsu and Ching-Hsiu Tsai. 2010. Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism. BMC Plant Biology, 10:286

  5. Huang, Y. W., Hu, C. C., Lin, N. S., and Hsu, Y. H. 2010. Mimicry of molecular pretenders: The terminal structures of satellites associated with plant RNA viruses. RNA Biol. 7, 162-171.

  6. Hu, C. C., Hsu, Y. H., and Lin, N. S. 2009. Satellite RNAs and Satellite Viruses of Plants. Viruses. 1, 1325-1350; doi:10.3390/v1031325.

  7. Liao, J. Y., Hu, C. C., Chen, C. C., Chang, C. H., and Deng, T. C., 2009. Full-length sequence analysis of a distinct isolate of Bidens mottle virus infecting sunflower in Taiwan. Arch. Virol. 154, 723-725.

  8. Huang, Y.W., Hu, C.C., Lin, C.A., Liu, Y.P., Tsai, C.H., Lin, N,S., Hsu, Y.H., 2009. Structural and functional analyses of the 3' untranslated region of Bamboo mosaic virus satellite RNA. Virology. 386, 139-153.

  9. Huang, Y.W., Hu, C.C., Lin, N.S., Tsai, C.H., Hsu, Y.H.. 2008. In vitro replication of Bamboo mosaic virus satellite RNA. Virus Res. 136, 98-106

  10. Shien , J.H., Wang, Y.S., Chen, C.H., Shieh, H.K., Hu, C,C., Chang, P.C., 2008. Identification of sequence changes in live attenuated goose parvovirus vaccine strains developed in Asia and Europe. Avian Pathol. 37,499-505

  11. Wu, C.Y., Lai, Y.C., Lin, N.S., Hsu, Y.H., Tsai, H. T., Liao, J. Y., and Hu, C.C., 2008. A simplified method of constructing infectious clones of begomovirus employing limited restriction enzyme digestion of products of rolling circle amplification. J. Virol. Methods 147, 355-359.

  12. Wu, C.Y., Yang, S.H., Lai, Y.C., Lin, N.S., Hsu, Y.H. and Hu, C.C., 2007. Unit-length, single-stranded circular DNAs of both polarity of begomoviruses are generated in Escherichia coli harboring phage M13-cloned begomovirus genome with single copy of replication origin. Virus Res. 125, pp. 14-28.

  13. Wu, J.-R., Shien, J.-H., Shieh, K.H., Hu, C.C., Gong, S.-R., Chen, L.-Y. and Chang, P.-C., 2007. Cloning of the gene and characterization of the enzymatic properties of themonomeric alkaline phosphatase (PhoX) from Pasteurella multocida strainX-73. FEMS Microbiol. Lett. 267, 113-120.

  14. Liao, J Y. Hu, C. C., Kao, J. L., and Deng, T.-C. 2007.  Identification of Tobacco mosaic virus infecting Rehmannia glutinosa .  Plant Pathol. Bull. 16, 61-69.

  15. Liao, J. Y., Hu, C. C., Lin, T. K., Chang, C. A., and Deng, T. C. 2007.  Identification of Squash leaf curl Philippines virus on Benincasa hispida in Taiwan.  Plant Pathol. Bull. 16, 11-18.

  16. Hajimorad, M. R., Hu, C. C., and Ghabrial, S. A. 1999. Molecular characterization of an atypical Old World strain of peanut stunt virus. Arch. Virol. 144: 1587-1600

  17. Chen, C. C. and Hu, C. C. 1999. Purification and characterization of a cucumovirus from Lisianthus rusellianus. Plant Prot. Bull. 41: 179-198.

  18. Di, R,. Hu, C.-C and Ghabrial, S. A. 1999. Complete nucleotide sequence of bean pod mottle comovirus RNA1: sequence comparisons and evolutionary relationships to other comoviruses. Virus Genes 18, 129-137.SCI

  19. Hu, C. C., and Ghabrial, S. A. (1998). Molecular evidence that strain BV-15 of peanut stunt cucumovirus is a reassortant between subgroup I and II strains. Phytopathology 88: 92-97SCI

  20. Hu, C. C., Sanger, M., and Ghabrial, S. A. 1998. Production of infectious RNA transcripts from full-length cDNA clones representing two subgroups of peanut stunt virus strains: mapping satellite RNA support to RNA1. J. Gen. Virol. 79, 2013-2021.SCI

  21. Hu, C. C., Sanger, M. P., and S. A. Ghabrial. (1999). A novel defective RNA associated with peanut stunt virus strain B. (manuscript in preparation)

  22. Hu, C. C., Aboul-Ata, A. E., Naidu, R. A., and Ghabrial, S. A. (1997). Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of RNA3. J. Gen. Virol. 78: 929-939.SCI

  23. Naidu, R. A., Hu, C. C., Pennington, R. E., and Ghabrial, S. A. (1995). Differentiation of eastern and western strains of peanut sutnt cucumovirus based on satellite RNA support and nucleotide sequence homology. Phytopathology 85: 502-507.SCI

  24. Hu, C. C., and Ghabrial, S. A. (1995). The conserved, hydrophilic and arginine-rich N-terminal domain of cucumovirus coat proteins contributes to their anomalous electrophoretic mobilities in sodium dodecylsulfate-polyacrylamide gels. J. Virol. Methods 55: 367-379.SCI

  25. Hsu, Y. H., Wu, C. W., Lee, C. W., Hu, C. C., and Lin, F. Z. (1994). Satellite defense as a control strategy for cucumber mosaic virus. Plant Pathol. Bull. 3: 72-77.820418B005091B03

  26. Lin, N. S., Lin, B. Y., Lo, N. W., Hu, C. C., Chow, T. Y., and Hsu, Y. H. (1994). Nucleotide sequence of the genomic RNA of bamboo mosaic potexvirus. J. Gen. Virol. 75: 2513-2518.SCI

  27. Hsu, Y. H., Lin, F. Z., Hu, C. C., and Yin, S. C. (1989). Host reaction, serology and RNA pattern of cucumber mosaic virus isolates. Plant Brot. Bull. 31:51-59.

  28. Hsu, Y. H., Hu, C. C., Lin, N. S., and Chiu, R. J. (1988). Symptom determinant of two Taiwan strains of cucumber nmosaic virus is on RNA3. Bot. Bull. Acad. Sinica. 29: 231-237.760201b00511
Ｂ、研討會論文：
  1. Chen, C. C., Hu, C. C., and Hsu, H. T. 2001. Characterization of a fabavirus isoated from diseased lisianthus. APS Annual Meeting.

  2. Ghabrial, S. A., Hu, C. C., Wright, P. W., Sanger, M. 1999. A novel defective RNA associated with peaut stunt cucumovirus (PSV) infections. APS Annual Meeting.

  3. Hu, C. C., and Ghabrial, S. A. (1993). Factors contributing to the anomalous electrophoretic mobility of cucumovirus coat proteins in SDS-polyacrylamide gels. Phytopathology 83, 1374

  4. Hu, C. C., and Ghabrial, S. A. (1996). Molecular characterization of peanut stunt virus (PSV) strain BV-15, a natural reassortant between subgroups I and II of PSV strains. APS/MSA joint annual meeting, Indianapolis, Indiana, 17

  5. Hu, C. C., and Ghabrial, S. A. (1995). The eastern and western strains of peanut stunt virus (PSV) represent two distinct subgroups based on serology and sequence homology. Phytpathology 85, 1152 
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