图片Dr. Wang, Min-Ying
Wang, Min-Ying

王敏盈 教授

Email: [email protected]

地址:中興大學食品暨生物科技大樓7F

Office phone:22840328 ext.761

Lab phone:22840328 ext.763

研究專長

蛋白質生產與純化、生物化學工程

學經歷

  • B.S. 國立台灣大學化工系, 1986
  • M.S. 國立台灣大學生化所, 1990
  • Ph.D. University of Maryland at College Park, l994
  • Post-Doctoral National Institutes of Health, l994-1995
  • 副教授 國立中興大學生物科技所, 1995-2003
  • 教授 國立中興大學生物科技所, 2003-present

主要教授課程 (含大學部及研究所)

  • 生物技術特論
  • 生物化學
  • 生化工程概論
  • 高等生化工程

研究興趣

       本實驗室的目標是利用昆蟲細胞/ 桿狀病毒表達系統（Insect cell culture/ baculovirus expression system）來生產似病毒粒子 (Virus-like particles, 簡稱 VLPs) 當作防治雞傳染性華氏囊病的疫苗，雞傳染性華氏囊病是由傳染性華氏囊病毒 (Infectious Bursal Disease Virus, 簡稱 IBDV) 所引起。

      之所以利用似病毒粒子而不用單一次單元蛋白的原因是似病毒粒子通常才能保有真正病毒原本之空間立體結構且誘發高titer 的中和抗體，是比較好的疫苗；而且這些似病毒粒子本身又不帶有 DNA 或 RNA 等遺傳性物質，就此點而言，發展似病毒粒子當作疫苗，又比用馴化活毒疫苗或死毒疫苗來得安全。

      以似病毒粒子當作疫苗是未來疫苗發展的一重要方向，我們將有系統兼定量性研究發展似病毒粒子之生產，及如何形成、如何簡易其純化步驟。

      IBDV 是影響全世界養雞產業經濟效益的主要病源體，所以它是一個非常好的模型系統，以後我們會朝其他疫苗發展(如C型肝炎疫苗)，以增強台灣疫苗開發及生產能力。 

期刊論文

  1. Meng-Chuan Wu, John Jaime Perez Coca, Gary Ro-Lin Chang, Shing-Yi Suen, Chia-Feng Lin, Hong-Nong Chou, Su-Yuan Lai and Min-Ying Wang*, 2012. "Chemical Modification of a Diatom’s Frustules for Protein and Viral Nanopartcile Adsorption" submitted to Process Biochemistry.

  2. Tsung-Hsien Chen, Ten-Hong Chen, Chung-Chi Hu, Jia-Teh Liao, Chin-Wei Lee, Jiunn-Wang Liao, Maw-Yeong Lin, Hung-Jen Liue, Min-Ying Wang, Na-Sheng Lin,f, Yau-Heiu Hsu, 2012 “Induction of protective immunity in chickens immunized with plant-made chimeric Bamboo mosaic virus particles expressing very virulent Infectious bursal disease virus antigen” Virus Research (accepted)

  3. Gary Ro-Lin Chang＃, Min-Ying Wang＃, Jiahn-Haur Liao, Yu-Ping Hsiao, Che-Wei Lee and Su-Yuan Lai*, 2011. “E. coli-derived VP4 Tubules of Infectious Bursal Disease Virus Have an Endopeptidase Activity” submitted to Process Biochemistry.

  4. Gary Ro-Lin Chang, Su-Yuan Lai, Poa-Chun Chang and Min-Ying Wang*, 2011. " Production of Immunogenic One-Component Avian H7-Subtype Influenza Virus-like Particles " Process Biochemistry 46:1292-1298 (SCI, Impact factor (2010): 2.648, Engineering, chemical, 17/135) 計畫編號: Grant No. NSC95-2313-B-005-036-MY2.

  5. Meng-Shiou Lee, Yi-Chiu Lin, Guan-Hua Lai, Su-Yaun Lai, Hsi-Jien Chen, Min-Ying Wang*, 2011. “One-step reverse transcription loop-mediated isothermal amplification for detection of infectious bursal disease virus.” Canadian Journal of Veterinary Research 75(2): 122-127 (SCI, Impact factor (2010):1.294, VETERINARY SCIENCES, 40/145) 計畫編號: Grant No. NSC97-2622-E-005-007-CC3, NSC 97-2313-B-005-009-MY3.

  6. Jin-Yi Ho, Long-Huw Lee, Yu-Chiang Lin, Yu-Jui Tai, Cheng-Kai Chang, Yu-mei Chou, Su-Yuan Lai and Min-Ying Wang*, 2010. "Vaccine Development through Terminal Deletions of an Infectious Bursal Disease Virus Protein 2 Precursor Variant" Process Biochemistry 45:786-793 (SCI, Impact factor (2010): 2.648, Engineering, chemical, 17/135) 計畫編號: Grant No. NSC90-2317-B-005-001 and NSC92-2313-B-005-119.

  7. Shwu-Jinng Wang, Wei-Feng Chang, Min-Ying Wang, Kuang-Pin Hsiung, Yung-Chuan Liu*, 2008. “Development of a disperse dye immunochromatographic test for the detection of antibodies against infectious bursal disease virus.” Veterinary Immunology and Immunopathology (125:284-290). (SCI, Impact factor (2010):2.176,VETERINARY SCIENCES, 13/141)  

  8. Min-Ying Wang, Hui-Ling Hu, Shing-Yi Suen, Fang-Yi Chiu, Jui-Hung Shien, and Su-Yuan Lai*, 2008. “Development of an Enzyme-linked Immunosorbent Assay for Detecting Infectious Bursal Disease Virus (IBDV) Infection Based on the VP3 Structural Protein.” Veterinary Microbiology 131:229-236. (SCI, Impact factor (2010): 3.256,VETERINARY SCIENCES, 3/145)

  9. Doong, Shyue-Ru; Chen, Yi-Huei; Lai, Su-Yuan; Lee, Cheng-Chung; Lin, Yu-Chiang; Min-Ying Wang*, 2007. "A Strong and Heterogeneous Adsorption of Infectious Bursal Disease VP2 Subviral Particle with Immobilized Metal Ions Depends on Two Surface Histidine Residues" Analytical Chemistry. 79:7654-7661. (SCI, Impact factor (2010): 5.874,CHEMISTRY, ANALYTICAL, 3/73)

  10. Ta-Wei Lin, Chi-Wen Lo, Su-Yuan Lai, Ruey-Jane Fan, Chao-Jung Lo, Yu-mei Chou, Rekha Thiruvengadam, Andrew H.-J. Wang and Min-Ying Wang*, 2007. “Chicken Heat Shock Protein 90 Is a Component of the Putative Cellular Receptor Complex of Infectious Bursal Disease Virus” Journal of Virology. 81:8730-8741. (SCI, Impact factor (2010):5.189,VIROLOGY, 5/33) 計畫編號: NSC 93-2313-B-005-070 to M.-Y.W. and NSC 92-2751-B-001-017-Y to A.H.-J.W. and M.-Y.W.)

  11. Cheng-Chung Lee, Tzu-Ping Ko, Chia-Cheng Chou, Masato Yoshimura, Shyue-Ru Doong, *Min-Ying Wang, and Andrew H.-J. Wang, 2006. “Crystal structure of infectious bursal disease virus VP2 subviral particle at 2.6 Å resolution: implications on virion assembly and immunogenicity” Journal of Structural Biology 155:74-86. (SCI, Impact factor (2010): 3.5, Biophysics, 26/73)

  12. Hui-Ling Hu, Min-Ying Wang, Chiung-Hsuah Chung, Shing-Yi Suen, 2006. “Purification of VP3 protein of Infectious Bursal Disease Virus Using Nickel Ion-Immobilized Regenerated Cellulose-Based Membranes” Journal of Chromatography B 840:76-84. (SCI, , Impact factor (2009): 2.777, Chemistry, analytical, 14/70)

  13. Meng-Shiou Lee, Jin-Yi Ho, Su-Yuan Lai, Shyue-Ru Doong, and *Min-Ying Wang, 2006. “Processing of Infectious Bursal Disease Virus (IBDV) and self-assemblies of IBDV-like Particles in Hi-5 Cells.” Biotechnology Progress 22:763-769. (SCI, Impact factor (2009):2.398, Food Science&Technology, 16/118)

  14. Chien-Shiuh Chen, Shing-Yi Suen, Su-Yuan Lai, Gary Ro-Lin Chang, Tsung-Chi Lu, Meng-Shiou Lee, and *Min-Ying Wang, 2005. “Purification of Capsid-like Particles of Infectious Bursal Disease Virus (IBDV) VP2 Expressed in E. coli with a Metal-ion Affinity Membrane System.” Journal of Virological Methods 130:51-58. (SCI, Impact factor (2009):2.133, Biotechnology&Applied Microbiology, 67/150) 計畫編號: NSC90-2317-B-005-001 and NSC92-2313-B-005-119.

  15. Meng-Shiou Lee, Min-Ying Wang, Yu-Jui Tai, and Su-Yuan Lai, 2004. “Characterization of particles formed by the precursor protein VPX of infectious bursal disease virus in insect Hi-5 cells: implication on its proteolytic processing” Journal of Virological Methods 121:191-199. (SCI, Impact factor (2009):2.133, Biotechnology&Applied Microbiology, 67/150) 計畫編號: NSC 89-2317-B-005-007 and NSC90-2317-B-005-001.

  16. Lai, S.-Y., Chen, H.-C., Lee, M.-S., Jinn, T.-R., Kao, S.-S. and *Wang, M.-Y., 2004. “Production and purification of immunogenic virus-like particles formed by the chimeric infectious bursal disease virus structural protein, rVP2H, in insect larvae” Process Biochemistry 39:571-577. (SCI, Impact factor (2009): 2.444, Engineering, chemical, 18/126) 計畫編號: NSC 89-2317-B-005-007.

  17. Lee, C.-C., Ko, T.-P., Lee, M.-S., Chou, C.-C., Lai, S.-Y., Wang, A. H.-J. and *Wang, M.-Y., 2003. “Purification, Crystallization, and Preliminary X-ray Analysis of Immunogenic Virus-Like Particles Formed by Infectious Bursal Disease Virus (IBDV) Structural Protein VP2” Acta Crystallographica Section D Biological Crystallography 59: 1234-1237. (SCI, Impact factor (2009):2.257 CRYSTALLOGRAPHY, 6/25) 計畫編號: NSC 90-2317-B-005-001 and NSC 89-2317-B-005-016.

  18. Tsai, Y.-H., Wang, M.-Y., Suen, S.-Y. 2002. “Purification of Hepatocyte Growth Factor Using PVDF-Based Immobilized Metal Affinity Membranes – Equilibrium Adsorption Study” Journal of Chromatography B: Biomedical Sciences & Applications. 766:133-143. (SCI, Impact factor (2009):2.777, Chemistry, analytical, 14/70)

  19. 李孟修、何靜宜、張丁榮、王守良、王敏盈。2002，利用昆蟲細胞表現外源蛋白，化工，第48卷第5期：82-96.

  20. Cheng, Y.-S., Lee, M.-S., Doong, S.-R. and Wang, M.-Y., 2001. “Separation of Pure and Immunoreactive Virus-Like Particles Using Gel-Filtration Chromatography Following Immobilized Metal Affinity Chromatography” Biotechnology Progress 17:318-325. (SCI) 計畫編號: NSC 88-2317-B-005-004 and NSC 89-2317-B-005-007.

  21. *Min-Ying Wang, Ya-Huey Yang, Hsuan-Shu Lee, and Su-Yuan Lai, 2000. “The Production of Functional Hepatocyte Growth Factor (HGF) in Insect Cells Infected with an HGF-recombinant Baculovirus in a Serum-Free Medium” Biotechnology Progress 16:146-151. (SCI) 計畫編號: NSC 87-2313-B-005-081.

  22. *Wang, M.-Y. and Doong, S.-R. 2000. “A pH-Based Fed-Batch Process for the Production of a Chimeric Recombinant Infectious Bursal Disease Virus (IBDV) Structural Protein (rVP2H) in Insect Cells” Process Biochemistry 35:877-884. (SCI) 計畫編號: NSC 88-2317-B-005-004.

  23. *Wang, M.-Y., Kuo, Y.-Y., Lee, M.-S., Doong, S.-R., Ho, J.-Y. and Lee, L.-H. 2000. “Self-Assembly of the Infectious Bursal Disease Virus Capsid Protein, rVP2, Expressed in Insect Cells and Purification of Immunogenic Chimeric rVP2H Particles by Immobilized Metal-Ion Affinity Chromatography” Biotechnology and Bioengineering 67:104-111. (SCI) 計畫編號: NSC 87-2313-B-005-081.

  24. Ho, J.-Y., Lai, S.-Y., Lee, L.-H. and *Wang, M.-Y. 1999 “Expression, Purification, and Characterization of the Infectious Bursal Disease Virus-Like Particles Produced by Insect Cells” Journal of the Chinese Chemical Society 46:743-750. (SCI) 計畫編號: 87-2313-B-005-081 and NSC 88-2317-B-005-004.

  25. Wang, S. L., Bentley, W. E., Liu, C. C., and *Wang, M. Y.1999 “The use of Glucose to Regulate pH Values of Culture Media and Increase the Production of Baculovirus (BmNPV) and Foreign Protein (HBsAg)” Process Biochemistry 34: 295-301. (SCI) 計畫編號: NSC-86-2316-B-005-001-BC.

  26. Lai, S.-Y., Ho, J.-Y. and *Wang, M. Y. 1998 “PCR method for detecting recombinant baculoviruses with the insertion of a large gene” Biotechnology Techniques  12: 733-736. (SCI) 計畫編號: NSC-87-2313-B-005-081

  27. Wang, S. L., Hou, R.F., *Wang, M. Y. 1997 “Factors Affecting Mass Production of HBsAg in M-BmN Cells Cultured in Shaking Flasks”, Chinese Journal of Entomology 17: 197-208 (in Chinese). 計畫編號: NSC-86-2316-B-005-001-BC

  28. Hu, Y. C., Wang, M. Y., and W. E. Bentley 1997 "A Segmented Flow Tubular Bioreactor for the Infection of Insect cells with Recombinant Baculovirus", Cytotechnology 24: 143-152. (SCI)

  29. Wang, M. Y., Kaslow, D. C. and Shiloach, J. 1996 “Production of Malaria Transmission-Blocking Vaccine from Recombinant Yeast”, Annals of the New York Academy of Sciences 782:123-132. (SCI)

  30. Wang, M. Y. and Bentley, W. E. 1996 “Kinetic Analysis of Protease, Recombinant Protein Production and Metabolites for Infected Sf-9 Cells under Different DO Levels”, Journal of Biotechnology 46:243-254. (SCI)

  31. Bentley, W. E., Kekbede, B., Franey, T., and Wang, M. Y. 1994 “Segregated Characterization of Recombinant Epoxide Hydrolase Synthesis via the Baculovirus/Insect Cell Expression System”, Chemical Engineering Science 49:4133. (SCI)

  32. Wang, M. Y. and Bentley, W. E. 1994 “Continuous Insect Cell (Sf-9) Culture with Aeration through Air Sparging”, Applied Microbiology and Biotechnology 41:317-413. (SCI)

  33. Wang, M. Y., Bentley, W. E. and Vakharia, V. 1994 “Purification of a Recombinant Protein Produced in a Baculovirus Expression System by Immobilized Metal Affinity Chromatography”, Biotechnology and Bioengineering 43:349-356. (SCI)

  34. Bentley, W. E., Wang, M. Y., Vakharia, V. 1994 “Development of an Efficient Bioprocess for Poultry Vaccines using High Density Insect Cell Culture”, Annals of the New York Academy of Sciences 745:336-359. (SCI)

  35. Wang, M. Y., Simon, K. and Bentley, W. E. 1993. “Effects of Oxygen/Glucose/Glutamine Feeding on Insect Cell Baculovirus Protein Expression: A Study on Epoxide Hydroxylase Production” Biotechnology Progress 9:355-361. (SCI)

  36. Wang, M. Y., Vakharia, V. and Bentley, W. E. 1993 “Synthesis of Epoxide Hydroxylase: A Focus on Infected Cells” Biotechnology and Bioengineering 42:240-247. (SCI)

  37. Wang, M. Y., Wester, K. and Bentley, W. E. 1993 “Glutamine Determination in Insect Cell Culture Media” Biotechnology Techniques 7(12): 841-846. (SCI)

實驗室簡介

  • 開發似病毒粒子當作疫苗
傳染性華氏囊病毒 (Infectious Bursal Disease Virus, 簡稱 IBDV)是影響全世界養雞產業經濟效益的主要病原體之一，近十年來更因高病源性毒株(very virulent strains)及變異株(variant strains) IBDV的出現及肆虐全球，使養雞工業大受影響而導致傳統之疫苗必須再重新開發。本實驗室已完成利用似病毒粒子(virus-like particles, VLPs)當作高效價性IBDV疫苗之開發，目前正執行一國家型應用研發計畫(雞傳染性華氏囊炎疫苗之開發)，目的是將似病毒粒子疫苗開發成具市場競爭力之疫苗。由於本研究成果深具商業化之潛力，所以本計畫有國內宏億生技公司(BioLeader; http://www.bioleader.com.tw)共同投資參與，我們相信本計畫之成功，對開發台灣動物疫苗的技術進展有相當大之助益。

  • 生物殺蟲劑之研發
另外，在環保意識抬頭的今日，生物技術防治害蟲被認為是取代濫用廣效性化學殺蟲劑的利器，在眾多利用生物技術防治害蟲的方法當中，昆蟲病毒之桿狀病毒對害蟲具有高致病力、高致死力、長儲存性及對環境安全等眾多優點，所以非常適合於做為害蟲防治。本實驗室自成立迄今，一直從事昆蟲細胞培養及病毒量產之相關研究，其中亦包含利用重組桿狀病毒生產外源蛋白。由於本實驗室對於昆蟲細胞培養方面的成就，所以興農公司(http://www.sinon.com.tw/)已與我們合作執行小產學計畫，利用該公司現有之發酵槽設備以培養昆蟲細胞、量產桿狀病毒，當作生物殺蟲劑用。

RESEARCH INTERESTS

  • Development of an Efficient Bioprocess for Vaccines usingInsect or Insect Cell Culture
The initial step of our research program will involve settingup a laboratory for insect cell culture and developing an efficientbioprocess for the production of recombinant protein using baculovirusexpression vectors. Due to the present situation in the biotechnologyindustry, research that focuses on the commercial development of biological and pharmaceutical products or academically interestedproteins, will drive the Department into a position of leadershipcapable of guiding biotechnology companies into large scale productionof useful products.

  • Development of an Understanding of Protein Separation using Metal-ionAffinity and Genetic Engineering Techniques
We are also interested in developing novel separation methodsfor recombinant protein from fermentation of cell culture. Sincecurrently available separation methods are high cost and low recovery,we try to develop a low cost, one-step purification method combiningthe techniques of genetic engineering and metal-ion affinity.In our previous research, a metal-ion binding domain was incorporatedinto a structural protein (VP2) from infectious bursal diseasevirus (IBDV) and this recombinant protein was purified by one-stepimmobilized metal-ion affinity chromatography. The next step to continue this work is by looking at the molecular interationsbetween the protein domain and metal ions in order to facilitatethe scale-up work.

  • Development of a Detailed Understanding of Viral-Infected CellMetabolism using Plug-Flow Bioreactor(PFBR) and a Model DescribingViral Growth
Infected cell metabolism is a very intensive research target,however, most of the studies are performed in batch culture. Theconditions in batch culture are dynamically changing, which doesnot provide a steady-state picture for viral infection and replicationnor facilitate the modeling work for viral growth and mutation.A two-stage bioreactor design combining a continuous stir tankbioreactor (CSTBR) for cell growth and a PFBR for viral infectionand replication is not only able to enhance the productivity ofrecombinant proteins, but also facilitate the modeling work.

國立中興大學生物科技學研究所© 2014　  [email protected] 　 TEL:(04)2284-0328　 FAX:(04)2285-3527
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